![]() ![]() Similar percent drops in metabolic activity of the iHCEC and pHCEC occurred after exposure to BAK, H2O2, or SDS, and the most significant changes in cytokine release occurred for IL-6 and IL-8. The damaging effects of UV on cell metabolic activity and cytokine release occurred at 5 min of UV exposure for iHCEC and 20 min for pHCEC. The release of inflammatory cytokines was measured using a multi-plex interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-alpha (TNF-α) assay, and cells were evaluated for viability using fluorescent dyes. Metabolic activity was measured using a metabolic assay. Cells were exposed to UV radiation and toxic doses of benzalkonium chloride (BAK), hydrogen peroxide (H2O2), and sodium dodecyl sulfate (SDS). ![]() This article describes the methods of measuring the toxicity of ultraviolet (UV) radiation and ocular toxins on primary (pHCEC) and immortalized (iHCEC) human corneal epithelial cell cultures. J., Subbaraman,L., Jones,L.ĭetermining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells Journal of Visualized Experiments 2021 173(July):e62675 Translational Relevance: This study showed that the novel, heated, in vitro blink model could be used to provide clinical insights into CL dehydration on the eye.Ĭhang,J. However, on the blink model, for most CLs, the WC significantly decreased after 1 hour but returned toward initial WC levels after 16 hours (P > 0.05).Ĭonclusions: The reduction in WC of CLs on the eye is likely due to both an increase in temperature and dehydration from air exposure and blinking. With the vial system, WC decreased and plateaued over time. Etafilcon A released more PHMB compared to all other lens types over a 24-hr period (p 0.05). After 3 months, Omega-3 Index increased by 34% in the treatment group (baseline, 5.3 ± 0.8 3 months, 8.0 ± 2.1 P 0.05). There were no significant changes in any of the ocular assessments at 1 or 3 months (all P >. Oxidative systems based on hydrogen peroxide or povidone-iodine showed a significant log10 reduction compared with the controls for both HCoV-229E and HCoV-OC43 in all tested conditions (all p 52 demonstrated an even larger significant improvement in symptoms with the treatment at 3 months compared with baseline (n = 13, −20.8 points, P =. For the 1% test, while Boston Simplus and OPTI-FREE exhibited a significant log10 reduction of both HCoV-229E (after 6 h) and HCoV-OC43 (after either 4 or 6 h incubation), those products showed less than 1 log10 reduction of the two infectious viruses. Ocular discomfort scores significantly exceeded baseline scores for 60 s following BD application, 120 s with OD, 135 s with BDdx, 150 s with ILL+, and 195 s with EC (all p 0.05) when 10% tests were performed. No inter-group differences in ocular parameters were noted at baseline (all p > 0.05). Visual acuity, non-invasive tear film stability, conjunctival hyperaemia, and ocular surface staining were assessed at baseline and 10 min. Participants rated subjective ocular discomfort during the 10-minute post-application period. On separate visits, spaced at least 48 h apart, participants were randomised to receive topical application of one of five eyelid cleansers or saline. Thirty healthy non-contact lens wearers (18 female mean ± SD age, 33 ± 12 years) were enrolled in a prospective randomised crossover study. To evaluate the short-term tolerability of five commercially available anti-demodectic eyelid cleansers OCuSOFT Oust Demodex (OD), I-MED I-Lid’n Lash Plus (ILL+), Labtician BlephaDex (BD), Chrissanthe Eye Cleanse (EC), and Théa Blephademodex (BDdx). Short-term tolerability of commercial eyelid cleansers: A randomised crossover study Contact Lens Anterior Eye 2022 Online ahead of print P., Bitton,E., Dantam,J., Jones,L., Ngo,W., Wang,m. UW School of Optometry & Vision ScienceĬraig,J.The cellular response to adverse conditions, including contact lens solutions and dry eyeīacterial binding and biofilm formation on contact lenses and other biomaterials.ĭry eye disease, tear film characterization and tissue assessment.Ĭontact lenses, spectacles and the use of objective vision assessments to predict subjective satisfaction. Myopia prevalence, correction and control. Investigating the anterior eye’s response to a range of conditions, in vivo, ex vivo and in vitro.ĭeveloping novel lens materials capable of delivering topical ophthalmic drugs to the eye. Protein and lipid quantification, determining protein activity and oxidized lipids, inflammatory marker analyses.ĭevelopment and characterization of biomaterials for a wide variety of anterior ocular applications.Ĭonnecting the dots between the day-to-day wearing experience, clinical signs and the characteristics of contemporary and future lens materials and solutions. Educational resources for practitioners. ![]()
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